Description
The Human IGF-I ELISA is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IGF-I in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for IGF-I coated on a 96-well plate. Standards and samples are pipetted into the wells and IGF-I present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and Biotinylated Anti-Human IGF-I antibody is added. After washing away unbound Biotinylated antibody, HRP-Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGF-I bound. The stop solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm